共1617篇 | 11月单细胞文献盘点

发表时间:2023-12-28 18:55作者:序科码

本期我们通过PubMed数据库检索,整理了从2023年11月1日到12月1日单细胞技术文章共1617篇。其中IF (影响因子)大于9分的文章有173篇(文章后面使用表格列出),现选择其中20篇代表性文章进行导读,详情如下:

01


英文题目:Brain-to-gut trafficking of alpha-synuclein by CD11c(+) cells in a mouse model of   Parkinson's disease
中文题目:帕金森病小鼠模型中CD11c ( + )细胞对α -突触核蛋白的脑-肠转运
发表时间:2023/11/20
发表杂志:Nature communications
影响因子:16.6
DOI:10.1038/s41467-023-43224-z

摘要:脑和肠道的炎症是一些神经系统疾病的重要组成部分,如帕金森病( Parkinson ' s disease,PD )。PD中免疫系统的触发因素之一是突触前蛋白α-同核蛋白(暂定名) (αSyn )的聚集。了解α Syn聚集体的传播机制对于开发疾病修饰疗法至关重要。利用脑优先的PD小鼠模型,我们证明了α Syn从雄性小鼠的大脑到回肠的转运。免疫组化显示回肠α Syn聚集物存在于CD11c +细胞中。通过单细胞RNA测序,我们证明了回肠CD11c +细胞是小胶质细胞样的,并且相同亚型的细胞在PD小鼠的大脑和回肠中被激活。此外,通过使用表达光转换蛋白Dendra2的小鼠,我们证明了CD11c +细胞从大脑运输到回肠。这些数据共同提供了α Syn在大脑和肠道之间运输的机制。

02

英文题目:CDC7 inhibition induces replication stress-mediated aneuploid cells with an   inflammatory phenotype sensitizing tumors to immune checkpoint blockade
中文题目:抑制CDC7可诱导具有炎症表型的复制应激介导的非整倍体细胞,使肿瘤对免疫检查点阻断敏感
发表时间:2023-11-18
发表杂志:Nat Commun
影响因子:16.6009
DOI:10.1038/s41467-023-43274-3

摘要丝氨酸/苏氨酸激酶,细胞分裂周期蛋白7 (CDC7)是启动DNA复制的关键。TAK-931是一种特异性CDC7抑制剂,是下一代复制应激(RS)诱导性因子。本研究对TAK-931的抗肿瘤疗效和免疫调节进行了临床前研究。TAK-931诱导RS,产生衰老样异倍体细胞,高表达炎性细胞因子和趋化因子(衰老相关分泌表型,SASP)。体内基因表达谱、免疫谱、免疫组织化学、RNA测序和单细胞RNA测序的多层组学分析显示,TAK-931产生的rs介导的非整倍体细胞强烈激活炎症相关和衰老相关通路,导致肿瘤浸润免疫细胞的聚集,产生强大的抗肿瘤免疫和疗效。最后,TAK-931和免疫检查点抑制剂联用显著增强了抗增殖活性。这些结果表明,TAK-931具有治疗性抗肿瘤特性,与常规免疫治疗联合应用可提高临床获益。

03

英文题目Characterization and decontamination of background noise in droplet-based single-cell protein expression data with DecontPro.

中文题目用DecontPro对基于液滴的单细胞蛋白质表达数据中的背景噪声进行表征和去污染。

发表时间:2023/11/17
发表杂志Nucleic acids research
影响因子:14.9
DOI:10.1093/nar/gkad1032

摘要诸如CITE-seq的分析可以使用抗体衍生标签(ADT)来测量单个细胞上的细胞表面蛋白的丰度。然而,许多ADT具有高水平的背景噪声,会使下游分析变得模糊不清。使用PBMC数据集的探索性分析,我们发现一些最初由于RNA水平低而被称为“空”的液滴包含高水平的ADTs,并可能对应于中性粒细胞。我们在空液滴中发现了一种新型的伪影,称为“海绵颗粒”,它具有中等水平的ADT表达,与环境噪声不同。海绵细胞中的ADT表达水平与几个数据集中真实细胞的背景峰中的ADT表达水平相关,表明它们可以与周围ADT一起构成背景噪声。然后,我们开发了DecontPro,这是一种新的贝叶斯分层模型,可以通过估计和消除这些来源的污染来净化ADT数据。在去除异常表达的ADT的同时保留天然ADT以及提高聚类特异性方面,DecontPro优于其他去污工具。总的来说,这些结果表明,应该对RNA和ADT数据分别进行空液滴鉴定,并且可以将DecontPro整合到CITE-seq工作流程中,以提高下游分析的质量。

04

英文题目:Charting the tumor microenvironment with spatial profiling technologies
中文题目:利用空间分析技术绘制肿瘤微环境图
发表时间:2023-12-9
发表杂志:Trends in cancer
影响因子:19.161
DOI:10.1016/j.trecan.2023.08.004

摘要:近年来,单细胞 RNA 测序 (scRNA-seq) 等能够实现细胞分辨率读数的技术提供了肿瘤微环境 (TME) 中细胞比例和表型的全面表征。然而,由于这些协议所需的样本解离步骤,它们无法捕获与细胞运行的复杂空间环境及其密集的相互作用网络相关的信息。空间分析技术最近已成为研究完整组织中 TME 细胞物理组织的一种有价值的方法。在这篇综述中,我们首先讨论空间分析技术如何推动 TME 表征,然后探讨其改善临床癌症患者诊断和预后的潜力。

05

英文题目:CHEX-seq detects single-cell genomic single-stranded DNA with catalytical potential

中文题目:CHEX-seq检测具有催化潜力的单细胞基因组单链DNA

发表时间:2023-11-14

发表杂志:Nature communications

影响因子:16.6
DOI:10.1038/s41467-023-43158-6

摘要:基因组DNA (gDNA)在转录、DNA修复和复制过程中发生单链和双链结构转换,这对维持细胞稳态至关重要。我们描述了在单个细胞中原位识别单链DNA (ssDNA)的“CHEX-seq”。CHEX-seq使用3 '末端阻断的光激活的探针启动ssDNA复制到测序的互补DNA中,从而报告全基因组单链染色质图谱。CHEX-seq以人类K562细胞为基准,并在小鼠和人类脑细胞的培养物以及脑切片中免疫染色的空间定位神经元中证明了其实用性。ssDNA的数量受扰动的动态调节。CHEX-seq还能识别单细胞中线粒体DNA的单链区域。令人惊讶的是,CHEX-seq在小鼠和人类gDNA中发现了体外催化卟啉金属化的单链位点,这表明基因组ssDNA具有催化活性。我们认为内源性DNA酶活性是基因组ssDNA的功能。

06

英文题目:Clinically relevant antibiotic resistance genes are linked to a limited set of taxa within gut microbiome worldwide

中文题目:临床上相关的抗生素耐药基因与全球肠道微生物组中的一组有限分类群有关

发表时间:2023-11-14
发表杂志:Nature communications
影响因子:16.6
DOI10.1038/s41467-023-42998-6

摘要:抗微生物药物耐药性(AR)基因的获得使重要病原体对抗生素几乎或完全无反应。有研究表明,病原体从肠道微生物群获得AR特征,而肠道微生物群是导致对所有类别抗生素耐药的AR基因的全球性储存库。然而,只有一部分AR基因导致对临床相关抗生素的耐药,尽管这些AR基因谱已经明确了常见病原体的特征,但我们对它们在肠道微生物群不同成员中的分类关联和转移潜力知之甚少。我们研究了来自33个国家的14,850个人类宏基因组和1,666个环境宏基因组,以及近600,000个分离株基因组,以深入了解临床相关AR基因的全球流行率和分类范围。我们发现,几个最令人关注的AR基因,如编码头孢菌素酶CTX-M和碳青霉烯酶KPC、IMP、NDM和VIM的基因,在分类上仍然局限于变形杆菌属。即使是人类肠道微生物组中最常见的碳青霉烯酶基因(cfiA),尽管是在一个可移动的质粒上发现,但仍然严格局限于拟杆菌属。我们使用高灵敏度单细胞融合PCR方法,在来自印度、洪都拉斯、巴基斯坦和越南的肠道微生物组样本中证实了这些发现。我们重点研究了一组编码碳青霉烯酶和头孢菌素酶的基因(迄今仅限于拟杆菌属),发现在另一个门中,几乎不需要突变就能发挥功能,这就提出了为什么这些基因没有更广泛地传播的问题。总体而言,这些数据表明,全球流行的、临床相关的AR基因尚未在不同的共生肠道微生物群中建立起来。

07

英文题目:Deciphering a critical role of uterine epithelial SHP2 in parturition initiation at single cell resolution

中文题目:破译子宫上皮SHP2在单细胞分辨率下分娩起始中的关键作用
发表时间:2023-11-14
发表杂志:Nature communications
影响因子:16.6
DOI:10.1038/s41467-023-43102-8

摘要:女性分娩的及时发生是怀孕成功的关键决定因素。高度异质性的母体蜕膜已越来越被认为是确定分娩时间的一个重要因素。尽管细胞类型在分娩中具有特定的作用,但子宫上皮在蜕膜中的作用仍然知之甚少。本研究通过COX1和COX2衍生的PGF2α利用上皮特异性SHP2敲除小鼠,揭示了上皮SHP2在分娩起始中的关键作用,其破坏有助于延迟分娩起始,难产和胎儿死亡。此外,我们还发现,在临近分娩的蜕膜中存在不同类型的上皮,单细胞分解伴随着通过增殖进行深刻的上皮改造。同时,上皮通过与间质细胞和巨噬细胞的交流来维持微环境。上皮微环境是由上皮细胞、间质细胞和子宫间质细胞的巨噬细胞密切相互作用维持的。简而言之,这项研究提供了以前未被重视的上皮在分娩准备中的作用,并阐明了早产的预防。

08

英文题目Early-to-mid stage idiopathic Parkinson's disease shows enhanced cytotoxicity and   differentiation in CD8 T-cells in females

中文题目:三峰单细胞分析揭示了一种新的儿科 CD8αα T 细胞亚群和整个 T 细胞区室的广泛年龄相关分子重编程
发表时间:2023-11-20
发表杂志:Nat Commun
影响因子:16.6
DOI:10.1038/s41467-023-43053-0

摘要大脑中的神经炎症是帕金森病 (PD) 发病机制的原因之一,但尚未系统地研究特发性 PD (iPD)的外周免疫潜在失调。在这里,我们展示了外周细胞毒性免疫环境升高,在分析8种先天性和适应性免疫特征后,早期至中度 iPD 患者(尤其是女性)新鲜血液中具有更多的终末分化效应记忆 (TEMRA) CD8 T、CD700 NKT 细胞和循环细胞毒性分子。这一特征也反映在较少的CD8FOXP3 T细胞上,在另一个亚队列中得到证实。CD8 TEMRA 和效应记忆 (TEM) 细胞中细胞毒性分子之间的共表达选择性增强。单细胞 RNA 测序分析表明,CD8 区室内加速分化,CD8 TEMRA 和 TEM 细胞的细胞毒性通路增强,而 CD8 中央记忆 (TCM) 和幼稚细胞已经更加活跃并转录重编程。我们的工作提供了iPD中外周免疫失调的综合图谱,提出了早期诊断和治疗的候选者。

09

英文题目:Expression Atlas update: insights from sequencing data at both bulk and single   cell level
中文题目:表达图谱更新:从整体和单细胞水平的测序数据的见解
发表时间:2023-11-22
发表杂志:Oxford
影响因子:14.9
DOI: 10.1093/nar/gkad1021

摘要:表达图谱(www.ebi.ac.uk/gxa)及其最新的单细胞表达图谱(www.ebi.ac.uk/gxa/sc)是EMBL-EBI的基因和蛋白质表达和定位知识库,用于批量和单细胞水平。这些资源的目的是允许用户研究它们在正常组织(基线)中的表达,或对疾病或基因型变化(差异)等扰动的反应。邀请用户在标准化的一致界面中跨物种或生物条件搜索基因或元数据术语。除了这些数据,单细胞表达图谱的新功能允许用户通过我们新的细胞类型轮搜索查询元数据。在实验层面,数据可以通过两种类型的降维图进行探索,t分布随机邻居嵌入(tSNE)和均匀流形逼近和投影(UMAP),覆盖聚类或元数据信息,以帮助用户理解。数据也被可视化为标记基因热图,识别有助于赋予集群身份的基因。对于一些数据,额外的可视化可作为交互式细胞水平解剖图和细胞类型基因表达热图。

10

英文题目: High-sensitive spatially resolved T cell receptor sequencing with SPTCR-seq
中文题目:利用SPTCR-seq进行高灵敏度空间分辨T细胞受体测序
发表时间:2023-10-18
发表杂志:Nat Commun
影响因子:16.6
DOI: 10.1038/s41467-023-43201-6

摘要:T细胞库的空间分辨率对于破译癌症相关的免疫功能障碍至关重要。目前的空间分辨转录组技术无法直接注释T细胞受体(TCR)。我们提出了空间分辨T细胞受体测序(SPTCR-seq),它集成了优化的靶富集和高灵敏度TCR测序的长读测序。SPTCR计算管道的产量和覆盖率可与单细胞TCR技术相媲美。我们将基于pcr的方法与SPTCR-seq方法进行比较,发现SPTCR-seq在重建整个TCR结构(包括V区、D区、J区和互补决定区3 (CDR3))方面具有优势。利用SPTCR-seq,我们评估了局部T细胞多样性和克隆扩展在空间离散的生态位。对肿瘤微环境和T细胞相互作用的探索揭示了NK细胞和B细胞在T细胞衰竭中的关键作用。整合空间分辨组学和TCR测序为探索癌症和其他疾病中的T细胞功能障碍提供了强大的工具。

11

英文题目:Mucosal-associated invariant T cells contribute to suppression of inflammatory   myeloid cells in immune-mediated kidney disease
中文题目:黏膜相关不变 T 细胞有助于抑制免疫介导的肾脏疾病中的炎性髓系细胞
发表时间:2023-11-15
发表杂志:Nature communications
影响因子:16.6
DOI:10.1038/s41467-023-43269-0

摘要:黏膜相关不变T(MAIT)细胞与屏障器官的各种炎症性疾病有关,但到目前为止,它们在肾脏疾病中的作用尚不清楚。在这里,我们报道了识别其原型配体的MAIT细胞,由MR1呈递的维生素B2中间体5-OP-RU,驻留在人和小鼠肾脏中。单细胞RNAseq分析揭示了几个肾内MAIT亚群,其中一个携带组织内MAIT17细胞的遗传指纹,在新月体肾小球肾炎(cGN)小鼠模型中被激活和扩增。在抗中性粒细胞胞浆抗体(ANCA)相关cGN患者的肾活检中也存在等效亚群。MAIT细胞缺陷的MR1小鼠表现出加重的疾病,而B6-MAIT投携带较高MAIT细胞数的小鼠受到cGN的保护。扩增的MAIT17细胞表达已知可抑制cGN的抗炎介质,例如CTLA-4、PD-1和TGF-β。相互作用组分析预测CXCR6-CXCL16介导的与肾单核吞噬细胞的串扰,已知可驱动cGN进展。同时,我们发现cGN在CXCL16阻断后加重。最后,我们提出了一种优化的5-OP-RU合成方法,并将其应用于减弱小鼠的cGN。总之,我们认为CXCR6 MAIT细胞可能在cGN中发挥保护作用,表明它们是抗炎治疗的潜在靶点。

12

英文题目:Mutation of key signaling regulators of cerebrovascular development in vein of   Galen malformations
中文题目:Galen 畸形静脉内脑血管发育关键信号调节因子的突变
发表时间:2023-11-17
发表杂志:Nat Commun.
影响因子:16.6
DOI:10.1038/s41467-023-43062-z

摘要:为了阐明 Galen 畸形(VOGM)是先天性脑动静脉畸形中最常见和最严重的静脉发病机制,我们对310个 VOGM 先证者家族外显子组和336,326个人脑血管单细胞转录组进行了综合分析。我们发现 Ras 抑制因子 p120 RasGAP (RASA1)具有全基因组显着的功能丧失从头变体负担(2042.5倍,p = 4.79 x 10-7)。Ephrin 受体 -B4(EPHB4)(17.5倍,p = 1.22 x 10-5)富集了罕见的破坏性传播变体,其与 p120 RasGAP 协同调节血管发育。其他先证者在 ACVRL1,NOTCH1,ITGB1和 PTPN11中有损伤性变异。ACVRL1变异也在多代 VOGM 家系中被鉴定出来。整合基因组分析确定发育中的内皮细胞可能是 VOGM 病理生理学的一个时空位点。表达 VOGM 特异性 EPHB4激酶结构域错义变体(Phe867Leu)的小鼠表现出破坏发育性血管生成和动脉-毛细血管-静脉网络的分层发育受损,但仅在存在“第二次打击”等位基因。这些结果阐明了人类动静脉发育和 VOGM 病理生物学,并对患者及其家属有一定意义。

13

英文题目:Plant Reactome Knowledgebase: empowering plant pathway exploration and OMICS data   analysis

中文题目:植物反应体知识库: 授权植物途径探索和 OMICS 数据分析
发表时间:2023-11-20
发表杂志:Nucleic Acids Res.
影响因子:14.9
DOI:10.1093/nar/gkad1052

摘要:植物反应组( https://plantreactome.gramene.org )是一个自由访问,全面的植物路径知识库。它提供了来自水稻(Oryza sativa)的策划的参考途径和基于基因整形学的途径投影到另外129个物种,跨越单细胞光自养生物,非维管植物和高等植物,从而包括广泛的分类多样性。目前,Plant Reactome 拥有339个参考通路,包括代谢和转运通路、激素信号传导、发育过程的遗传调控以及协调植物对非生物和生物刺激反应的复杂转录网络。Plant Reactome 不仅仅是一个存储库,它还是一个动态数据发现平台。用户可以分析和可视化组学数据,如基因表达,基因-基因相互作用,蛋白质组和代谢组数据,所有在丰富的背景下的植物途径。Plant Reactome 致力于促进数据互操作性,维护全球数据标准,并遵循可查找、可访问、互操作和可重用(FAIR)数据政策的原则。

14

英文题目:Protective effects of macrophage-specific integrin α5 in myocardial infarction   are associated with accentuated angiogenesis

中文题目巨噬细胞特异性整合素α5在心肌梗死中的保护作用与血管生成加剧有关

发表时间:2023/11/20
发表杂志:Nature communications
影响因子:16.6
DOI :10.1038/s41467-023-43369-x

摘要:巨噬细胞通过整合素感知细胞外基质环境的变化,并在调节心肌梗死后的修复反应中起核心作用。在这里,我们表明巨噬细胞整合素α5保护梗死心脏免受不良重塑,并且保护作用与血管生成巨噬细胞表型的获得有关。我们证明髓样细胞和巨噬细胞特异性整合素α5敲除小鼠加剧了梗死后不良的重塑,并伴有梗死和边界区的血管生成减少。单细胞 RNA 测序可鉴定具有高 Itga5 表达的血管生成性梗死巨噬细胞群。整合素α5在巨噬细胞中的血管生成作用涉及血管内皮生长因子A的上调,在体内和体外对巨噬细胞转录组进行RNA测序,然后进行生物信息学分析,将几种细胞内激酶确定为整合素α5的潜在下游靶标。中和试验表明,整合素 α5 刺激的巨噬细胞的血管生成作用涉及局灶粘附激酶和磷酸肌醇 3 激酶级联反应的激活。

15

英文题目scReadSim: a single-cell RNA-seq and ATAC-seq read simulator

中文题目:scReadSim:单细胞RNA-seq和ATAC-seq读取模拟器
发表时间:2023-11-18
发表杂志:Nat Commun
影响因子:16.6009
DOI10.1038/s41467-023-43162-w

摘要基准单细胞RNA-seq (scRNA-seq)和使用测序的转座酶可及染色质单细胞分析(scATAC-seq)计算工具要求模拟器生成真实的测序读长。然而,没有一个读取模拟器旨在模拟真实的数据。为了填补这一空白,我们引入了scReadSim,这是一种单细胞RNA-seq和ATAC-seq读取模拟器,它允许用户指定的真实数据,并通过模拟真实数据生成合成测序读取(在FASTQ或BAM文件中)。在读序列和读计数水平,scReadSim模拟了真实的scRNA-seq和scATAC-seq数据。此外,scReadSim提供了基本信息,包括scRNA-seq的唯一分子标识符(UMI)计数和scATAC-seq的开放染色质区域。特别是,scReadSim允许用户设计细胞类型特异性的ground-truth开放染色质区域,用于生成scATAC-seq数据。在scReadSim的基准应用中,我们表明,ui -tools在scRNA-seq UMI重复数据删除中达到了最高的准确性,HMMRATAC和MACS3在scATAC-seq峰值调用中达到了最高的性能。

16

英文题目:Simultaneous selection of nanobodies for accessible epitopes on immune cells in the tumor microenvironment

中文题目:同步选择纳米抗体用于肿瘤微环境中免疫细胞的可及表位
发表时间:2023-11-17
发表杂志Nat Commun
影响因子16.6009
DOI10.1038/s41467-023-43038-z

摘要在快速发展的合成生物学领域,迫切需要发现用于治疗性生物制剂的靶向部分的技术。在这里,我们提出了INSPIRE-seq,一种利用纳米抗体库和二代测序来识别复杂环境中选择的纳米抗体的方法。INSPIRE-seq使穿透肿瘤微环境的免疫细胞结合纳米抗体平行富集成为可能。肿瘤、淋巴结和脾脏中不同免疫细胞亚型的克隆富集和特异性不同。INSPIRE-seq识别了与PHB2结合的树突状细胞结合克隆。单细胞RNA测序揭示了与cDC1s的连接,免疫荧光证实了纳米抗体- phb2沿细胞膜共定位。结构建模和对接研究有助于结合预测,并将指导纳米抗体的选择。在这项工作中,我们证明了INSPIRE-seq提供了一种无偏倚的方法来检查复杂的微环境,并协助纳米抗体的开发,纳米抗体可以用作活性药物、修饰成药物或用作靶向部分。

17

英文题目: Single cell transcriptomics shows that malaria promotes unique regulatory responses across multiple immune cell subsets
中文题目:单细胞转录组学表明疟疾促进多个免疫细胞亚群的独特调节反应
发表时间:2023-11-15
发表杂志: Nat Commun
影响因子:17.694
DOI:10.1038/s41467-023-43181-7.

摘要:恶性疟原虫疟疾可驱动多个细胞亚群的免疫调节反应,从而防止免疫发病,但也阻碍了有效抗寄生虫免疫的发展。了解疟疾在特定细胞亚群中诱导的耐受性反应可能有助于制定药物治疗和疫苗接种期间增强保护性免疫力的策略。在这里,我们通过单细胞RNA 测序分析了恶性疟原虫疟疾期间的免疫状况。我们鉴定了亚簇主要免疫细胞类型中的细胞类型特异性反应。疟疾与免疫抑制性单核细胞的增加以及上调耐受性标记物的 NK 和 γδ T细胞有关。还诱导产生 IL-10 的 Tr1 CD4 T 细胞和产生 IL-10 的调节性 B 细胞。所有细胞类型均发现 I 型干扰素反应,这表明 I 型干扰素信号传导可能与疟疾期间免疫调节网络的诱导有关。这些发现提供了对疟疾期间细胞特异性和共同免疫调节变化的见解,并为进一步分析提供了数据资源。

18

英文题目:Single-cell epigenomics and spatiotemporal transcriptomics reveal human cerebellar development
中文题目:单细胞表观基因组学和时空转录组学揭示人类小脑发育
发表时间:2023-11
发表杂志:nature communications
影响因子:16.6
DOI:10.1038/s41467-023-43568-6

摘要:人类小脑发育由分子调控网络协调,以实现细胞结构并协调运动和认知功能。在这里,我们结合了单细胞转录组学,空间转录组学和单细胞染色质可及性状态,以系统地描绘人类胎儿小脑发育的整合时空景观。我们揭示了转录因子和顺式调节元件 (CREs) 的组合在控制祖细胞分化和细胞命运决定中发挥作用,以分层的方式沿着轨迹,为这些细胞提供细胞命运和空间信息的基因表达调控图。我们还说明了位于小脑皮质不同区域的颗粒细胞在发育过程中显示出由不同信号调节的不同分子特征。最后,我们绘制了与小脑功能障碍相关的疾病的单核苷酸多态性 (SNPs),并发现几种疾病相关基因仅在人类中显示出时空和细胞类型特异性表达模式,这表明了神经精神疾病发病的细胞基础和可能机制。

19

英文题目:Dissecting the human leptomeninges at single-cell resolution
中文题目: 灵长类动物大脑单细胞转录组比较分析揭示人类特异性调控进化
发表时间:2023-11-16
发表杂志:Nat Commun
影响因子: 16.6
DOI:10.1038/s41467-023-43218-x

摘要 :与所有远距离投射一样,视网膜病变可以由轴突本身或其靶标产生。然而,在哺乳动物大脑的细微尺度上,潜在的连接模式仍然难以捉摸。为了解决这个问题,我们使用体内双光子钙成像技术,在单细胞分辨率尺度,功能性地绘制了雌性小鼠视网膜-上丘通路中输入轴突和靶神经元的空间组织。我们发现视网膜神经节细胞轴突末端有近乎完美的视网膜专题平铺,平均误差低于 30微米或2°视角。上丘局部神经元的视网膜定位精度相对较低。随后的数据驱动建模将其归因于低输入收敛,上丘中每个突触后细胞平均有 5.5 个视网膜神经节细胞输入。这些结果表明,视网膜分布很大程度上源于突触前细胞的地形精确输入,而不是通过突触后细胞精心设计局部电路来重建地形。

20

英文题目:The transcriptional and phenotypic characteristics that define alveolar   macrophage subsets in acute hypoxemic respiratory failure

中文题目:在急性低氧性呼吸衰竭中定义肺泡巨噬细胞亚群的转录和表型特征
发表时间:2023-11-17
发表杂志:Nat Commun
影响因子:16.600
DOI:10.1038/s41467-023-43223-0

摘要:确定急性低氧性呼吸衰竭(AHRF)中肺泡单核细胞和巨噬细胞亚群的转录和表型特征尚不清楚。在本研究中,我们将CITE-seq(单细胞rna测序和细胞表面蛋白定量)应用于纵向收集的AHRF参与者的支气管肺泡灌洗液和血液样本,以识别肺泡髓系亚群,然后使用流式细胞术在外部队列中验证其身份。我们确定了与其他肺部疾病不同的转录谱肺泡髓系亚群,以及健康参与者(金属硫蛋白)或COVID-19患者(CD163/LGMN)中转录谱相似的几个亚群。我们使用来自CITE-seq的信息来确定区分转录亚群(CD14、CD163、CD123、CD71、CD48、CD86和CD44)的细胞表面蛋白。在外部队列中,我们发现较高比例的CD163/LGMN肺泡巨噬细胞与AHRF患者的死亡率相关。我们使用可扩展的方法(可应用于临床队列),报告了一组区分肺泡髓系亚群的细胞表面蛋白。

Title
Journal
Impact Factor
A single cell genomics atlas of the Drosophila larval eye reveals distinct   photoreceptor developmental timelines
Nat Commun
  16.600
A statistical framework for differential pseudotime analysis with multiple   single-cell RNA-seq samples
Nat Commun
  16.600
An Outer Membrane-Inspired Polymer Coating Protects and Endows Escherichia coli   with Novel Functionalities
Small
  13.300
Bioorthogonal Reaction-Mediated Enzymatic Elongation-Driven Dendritic   Nanoassembly for Genome-Wide Analysis of 5-Hydroxymethyluracil in Breast Tissues
Nano Lett
  10.800
BloodSpot 3.0: a database of gene and protein expression data in normal and   malignant haematopoiesis
Nucleic Acids Res
  14.900
Brain-to-gut trafficking of alpha-synuclein by CD11c(+) cells in a mouse model of   Parkinson's disease
Nat Commun
  16.600
CamoTSS: analysis of alternative transcription start sites for cellular   phenotypes and regulatory patterns from 5' scRNA-seq data
Nat Commun
  16.600
CAR(+) and CAR(-) T cells share a differentiation trajectory into an NK-like   subset after CD19 CAR T cell infusion in patients with B cell malignancies
Nat Commun
  16.600
CDC7 inhibition induces replication stress-mediated aneuploid cells with an   inflammatory phenotype sensitizing tumors to immune checkpoint blockade
Nat Commun
  16.600
Cellular age explains variation in age-related cell-to-cell transcriptome   variability
Genome Res
   7.000
Characterization and decontamination of background noise in droplet-based   single-cell protein expression data with DecontPro
Nucleic Acids Res
  14.900
Charting the tumor microenvironment with spatial profiling technologies
Trends Cancer
  18.400
CHEX-seq detects single-cell genomic single-stranded DNA with catalytical   potential
Nat Commun
  16.600
Clinically relevant antibiotic resistance genes are linked to a limited set of   taxa within gut microbiome worldwide
Nat Commun
  16.600
Coexistence of Multiple Functional Variants and Genes Underlies Genetic Risk   Locus 11p11.2 of Alzheimer's Disease
Biol Psychiatry
  10.600
Combining single-cell RNA sequencing and population-based studies reveals hand   osteoarthritis-associated chondrocyte subpopulations and pathways
Bone Res
  12.700
Comparative single-cell transcriptomic analysis of primate brains highlights   human-specific regulatory evolution
Nat Ecol Evol
  16.800
Correction to 'Cluster-independent marker feature identification from single-cell   omics data using SEMITONES'
Nucleic Acids Res
  14.900
Creation of a High-Throughput Microfluidic Platform for Single-Cell Transcriptome   Sequencing of Cell-Cell Interactions
Small Methods
  12.400
Cytolytic circumsporozoite-specific memory CD4(+) T cell clones are expanded   during Plasmodium falciparum infection
Nat Commun
  16.600
Dbh(+) catecholaminergic cardiomyocytes contribute to the structure and function   of the cardiac conduction system in murine heart
Nat Commun
  16.600
Deciphering a critical role of uterine epithelial SHP2 in parturition initiation   at single cell resolution
Nat Commun
  16.600
Deep-qGFP: A Generalist Deep Learning Assisted Pipeline for Accurate   Quantification of Green Fluorescent Protein Labeled Biological Samples in   Microreactors
Small Methods
  12.400
Dermis resident macrophages orchestrate localized ILC2 eosinophil circuitries to   promote non-healing cutaneous leishmaniasis
Nat Commun
  16.600
Detection of isoforms and genomic alterations by high-throughput full-length   single-cell RNA sequencing in ovarian cancer
Nat Commun
  16.600
Discovery and Validation of Novel Genes in a Large Chinese Autism Spectrum   Disorder Cohort
Biol Psychiatry
  10.600
DiSignAtlas: an atlas of human and mouse disease signatures based on bulk and   single-cell transcriptomics
Nucleic Acids Res
  14.900
Disrupting cellular memory to overcome drug resistance
Nat Commun
  16.600
Dissecting mammalian reproduction with spatial transcriptomics
Hum Reprod Update
  13.300
Dissecting the human leptomeninges at single-cell resolution
Nat Commun
  16.600
DRMref: comprehensive reference map of drug resistance mechanisms in human   cancer
Nucleic Acids Res
  14.900
Early-to-mid stage idiopathic Parkinson's disease shows enhanced cytotoxicity and   differentiation in CD8 T-cells in females
Nat Commun
  16.600
Explainable machine learning for profiling the immunological synapse and   functional characterization of therapeutic antibodies
Nat Commun
  16.600
Expression Atlas update: insights from sequencing data at both bulk and single   cell level
Nucleic Acids Res
  14.900
Expression profiling of cerebrospinal fluid identifies dysregulated antiviral   mechanisms in multiple sclerosis
Brain
  14.500
FiCRoN, a deep learning-based algorithm for the automatic determination of   intracellular parasite burden from fluorescence microscopy images
Med Image Anal
  10.900
Fragment-sequencing unveils local tissue microenvironments at single-cell   resolution
Nat Commun
  16.600
Germline PTEN genotype-dependent phenotypic divergence during the early neural   developmental process of forebrain organoids
Mol Psychiatry
  11.000
Glioblastoma revisited: from neuronal-like invasion to pacemaking
Trends Cancer
  18.400
Highly Multiplexed, Efficient, and Automated Single-Cell MicroRNA Sequencing with   Digital Microfluidics
Small Methods
  12.400
High-Precision Single-Cell microRNA Therapy by a Functional Nanopipette with   Sensitive Photoelectrochemical Feedback
Small
  13.300
High-sensitive spatially resolved T cell receptor sequencing with SPTCR-seq
Nat Commun
  16.600
IL-17 signalling is critical for controlling subcutaneous adipose tissue dynamics   and parasite burden during chronic murine Trypanosoma brucei infection
Nat Commun
  16.600
IL-21R-STAT3 signalling initiates a differentiation program in uterine   tissue-resident NK cells to support pregnancy
Nat Commun
  16.600
Immuno-metabolic dendritic cell vaccine signatures associate with overall   survival in vaccinated melanoma patients
Nat Commun
  16.600
Immunoprecipitation of RNA-DNA hybrid interacting proteins in Trypanosoma brucei   reveals conserved and novel activities, including in the control of surface   antigen expression needed for immune evasion by antigenic variation
Nucleic Acids Res
  14.900
Impedance-Based Multimodal Electrical-Mechanical Intrinsic Flow Cytometry
Small
  13.300
In situ visualization of m6A sites in cellular mRNAs
Nucleic Acids Res
  14.900
Inflammation in the tumor-adjacent lung as a predictor of clinical outcome in   lung adenocarcinoma
Nat Commun
  16.600
Integrating SNVs and CNAs on a phylogenetic tree from single-cell DNA sequencing   data
Genome Res
   7.000
Integrating spatial and single-cell transcriptomics data using deep generative   models with SpatialScope
Nat Commun
  16.600
Integrative analysis reveals a conserved role for the amyloid precursor protein   in proteostasis during aging
Nat Commun
  16.600
Label-Free Intracellular Multi-Specificity in Yeast Cells by Phase-Contrast   Tomographic Flow Cytometry
Small Methods
  12.400
Leveraging Single-Cell Populations to Uncover the Genetic Basis of Complex   Traits
Annu Rev Genet
  11.100
Mapping and modeling human colorectal carcinoma interactions with the tumor   microenvironment
Nat Commun
  16.600
Massively Parallel High-Throughput Single-Cell Patterning and Large Biomolecular   Delivery in Mammalian Cells Using Light Pulses
Small
  13.300
Microglia sense astrocyte dysfunction and prevent disease progression in an   Alexander disease model
Brain
  14.500
Microwell Confined Electro-Coalescence for Rapid Formation of High-Throughput   Droplet Array
Small
  13.300
Modular interneuron circuits control motion sensitivity in the mouse retina
Nat Commun
  16.600
Mouse models of pediatric high-grade gliomas with MYCN amplification reveal   intratumoral heterogeneity and lineage signatures
Nat Commun
  16.600
mtFociCounter for automated single-cell mitochondrial nucleoid quantification and   reproducible foci analysis
Nucleic Acids Res
  14.900
Mucosal-associated invariant T cells contribute to suppression of inflammatory   myeloid cells in immune-mediated kidney disease
Nat Commun
  16.600
Multiple parallel origins of parasitic Marine Alveolates
Nat Commun
  16.600
Mutation of key signaling regulators of cerebrovascular development in vein of   Galen malformations
Nat Commun
  16.600
Paired single-cell multi-omics data integration with Mowgli
Nat Commun
  16.600
PD-1(-) CD45RA(+) effector-memory CD8 T cells and CXCL10(+) macrophages are   associated with response to atezolizumab plus bevacizumab in advanced   hepatocellular carcinoma
Nat Commun
  16.600
Plant Reactome Knowledgebase: empowering plant pathway exploration and OMICS data   analysis
Nucleic Acids Res
  14.900
Pooled Genome-Scale CRISPR Screens in Single Cells
Annu Rev Genet
  11.100
Protective effects of macrophage-specific integrin 伪5 in myocardial infarction   are associated with accentuated angiogenesis
Nat Commun
  16.600
Recent Progress in Mass Spectrometry-Based Single-Cell Metabolic Analysis
Small Methods
  12.400
Roles of bone morphogenetic proteins in endometrial remodeling during the human   menstrual cycle and pregnancy
Hum Reprod Update
  13.300
SCAN: Spatiotemporal Cloud Atlas for Neural cells
Nucleic Acids Res
  14.900
scDREAMER for atlas-level integration of single-cell datasets using deep   generative model paired with adversarial classifier
Nat Commun
  16.600
scReadSim: a single-cell RNA-seq and ATAC-seq read simulator
Nat Commun
  16.600
Shortcut barcoding and early pooling for scalable multiplex single-cell   reduced-representation CpG methylation sequencing at single nucleotide   resolution
Nucleic Acids Res
  14.900
Simultaneous selection of nanobodies for accessible epitopes on immune cells in   the tumor microenvironment
Nat Commun
  16.600
Single cell spatial analysis reveals inflammatory foci of immature neutrophil and   CD8 T cells in COVID-19 lungs
Nat Commun
  16.600
Single cell transcriptomics shows that malaria promotes unique regulatory   responses across multiple immune cell subsets
Nat Commun
  16.600
Single Nanovesicles Tracking Reveals Their Heterogeneous Extracellular   Adsorptions
Small
  13.300
Single-cell analysis identifies genes facilitating rhizobium infection in Lotus   japonicus
Nat Commun
  16.600
Single-cell epigenomics and spatiotemporal transcriptomics reveal human   cerebellar development
Nat Commun
  16.600
Single-cell transcriptomics reveals the brain evolution of web-building spiders
Nat Ecol Evol
  16.800
SMARCB1 loss activates patient-specific distal oncogenic enhancers in malignant   rhabdoid tumors
Nat Commun
  16.600
Spatial transcriptomic interrogation of the murine bone marrow signaling   landscape
Bone Res
  12.700
Spatial transcriptomics deconvolution at single-cell resolution using Redeconve
Nat Commun
  16.600
SPDB: a comprehensive resource and knowledgebase for proteomic data at the   single-cell resolution
Nucleic Acids Res
  14.900
StemDriver:聽a knowledgebase of gene functions for hematopoietic stem cell fate   determination
Nucleic Acids Res
  14.900
STOmicsDB: a comprehensive database for spatial transcriptomics data sharing,   analysis聽and visualization
Nucleic Acids Res
  14.900
TBtools-II: A "one for all, all for one" bioinformatics platform for biological   big-data mining
Mol Plant
  27.500
The effects of methylphenidate and atomoxetine on Drosophila brain at single-cell   resolution and potential drug repurposing for ADHD treatment
Mol Psychiatry
  11.000
The extrafollicular B cell response is a hallmark of childhood idiopathic   nephrotic syndrome
Nat Commun
  16.600
The P10K database: a data portal聽for the protist 10 000 genomes project
Nucleic Acids Res
  14.900
The transcriptional and phenotypic characteristics that define alveolar   macrophage subsets in acute hypoxemic respiratory failure
Nat Commun
  16.600
Topographic axonal projection at single-cell precision supports local retinotopy   in the mouse superior colliculus
Nat Commun
  16.600
Vector-borne Trypanosoma brucei parasites develop in artificial human skin and   persist as skin tissue forms
Nat Commun
  16.600
ZEBRA: a hierarchically integrated gene expression atlas of the murine and human   brain at single-cell resolution
Nucleic Acids Res
  14.900
A computer vision and residual neural network (ResNet) combined method for   automated and accurate yeast replicative aging analysis of high-throughput   microfluidic single-cell images
Biosens Bioelectron
  12.600
A k-mer-Based Approach for Phylogenetic Classification of Taxa in Environmental   Genomic Data
Syst Biol
   6.500
A reversible epigenetic memory of inflammatory injury controls lineage plasticity   and tumor initiation in the mouse pancreas
Dev Cell
  11.800
A single-nucleus transcriptomic atlas of primate testicular aging reveals   exhaustion of the spermatogonial stem cell reservoir and loss of Sertoli cell   homeostasis
Protein Cell
  21.100
AAV vectors displaying bispecific DARPins enable dual-control targeted gene   delivery
Biomaterials
  14.000
Alpha-1 adrenergic signaling drives cardiac regeneration via extracellular matrix   remodeling transcriptional program in zebrafish macrophages
Dev Cell
  11.800
An end-to-end workflow for multiplexed image processing and analysis
Nat Protoc
  14.800
Analyzing somatic mutations by single-cell whole-genome sequencing
Nat Protoc
  14.800
Annotation of cell types (ACT): a convenient web server for cell type annotation
Genome Med
  12.300
Bacteria detection and species identification at the single-cell level using   super-resolution fluorescence imaging and AI analysis
Biosens Bioelectron
  12.600
Biosensors in microalgae: A roadmap for new opportunities in synthetic biology   and biotechnology
Biotechnol Adv
  16.000
Combined inactivation of RB and Hippo converts differentiating Drosophila   photoreceptors into eye progenitor cells through derepression of homothorax
Dev Cell
  11.800
ConDoR: tumor phylogeny inference with a copy-number constrained mutation loss   model
Genome Biol
  12.300
Confinement-enhanced microalgal individuals biosensing for digital atrazine   assay
Biosens Bioelectron
  12.600
Control of murine brown adipocyte development by GATA6
Dev Cell
  11.800
Cytomulate: accurate and efficient simulation of CyTOF data
Genome Biol
  12.300
Defining the identity and the niches of epithelial stem cells with highly   pleiotropic multilineage potency in the human thymus
Dev Cell
  11.800
Evaluation of deep learning-based feature selection for single-cell RNA   sequencing data analysis
Genome Biol
  12.300
Ferroptosis-enhanced chemotherapy for triple-negative breast cancer with magnetic   composite nanoparticles
Biomaterials
  14.000
Genomic and transcriptomic advances in amyotrophic lateral sclerosis
Ageing Res Rev
  13.100
GITR and TIGIT immunotherapy provokes divergent multicellular responses in the   tumor microenvironment of gastrointestinal cancers
Genome Med
  12.300
Inferring cellular and molecular processes in single-cell data with non-negative   matrix factorization using Python, R and GenePattern Notebook implementations of   CoGAPS
Nat Protoc
  14.800
Integrating single-cell imaging and RNA sequencing datasets links differentiation   and morphogenetic dynamics of human pancreatic endocrine progenitors
Dev Cell
  11.800
INVADEseq to identify cell-adherent or invasive bacteria and the associated host   transcriptome at single-cell-level resolution
Nat Protoc
  14.800
Loss of p53-DREAM-mediated repression of cell cycle genes as a driver of lymph   node metastasis in head and neck cancer
Genome Med
  12.300
Maternal and embryonic signals cause functional differentiation of luminal   epithelial cells and receptivity establishment
Dev Cell
  11.800
Mesenchymal stem cell attenuates spinal cord injury by inhibiting mitochondrial   quality control-associated neuronal ferroptosis
Redox Biol
  11.400
Microbial gas fermentation technology for sustainable food protein production
Biotechnol Adv
  16.000
Multi-scale characterisation of homologous recombination deficiency in breast   cancer
Genome Med
  12.300
Neural stem cells persist to generate new neurons in the hippocampus of adult and   aged human brain - Fiction or accurate?
Ageing Res Rev
  13.100
Neutrophil and NETosis Modulation in Traumatic Heterotopic Ossification
Ann Surg
   9.000
Optical-based microbubble for on-demand droplet release from static droplet array   (SDA) for dispensing one droplet into one tube
Biosens Bioelectron
  12.600
Personalized tumor combination therapy optimization using the single-cell   transcriptome
Genome Med
  12.300
Reconstructing human brown fat developmental trajectory in聽vitro
Dev Cell
  11.800
RNA methylation-driven assembly of fluorescence-encoded nanostructures for   sensitive detection of m(6)A modification writer METTL3/14 complex in human   breast tissues
Biosens Bioelectron
  12.600
Scalable single-cell profiling of chromatin modifications with sciCUT&Tag
Nat Protoc
  14.800
Single bacteria identification with second-harmonic generation in MoS(2)
Biosens Bioelectron
  12.600
Single-cell analysis of shared signatures and transcriptional diversity during   zebrafish development
Dev Cell
  11.800
Single-cell multiomics of the human retina reveals hierarchical transcription   factor collaboration in mediating cell type-specific effects of genetic variants   on gene regulation
Genome Biol
  12.300
Single-cell RNA sequencing of intestinal crypts reveals vital events in damage   repair and the double-edged sword effect of the Wnt3/尾-catenin pathway in   irradiated mice
Redox Biol
  11.400
Single-cell RNA-seq of maize meiocytes and pollen grains
Nat Protoc
  14.800
Skeletal muscle regeneration failure in ischemic-damaged limbs is associated with   pro-inflammatory macrophages and premature differentiation of satellite cells
Genome Med
  12.300
Transcription factor NFYa controls cardiomyocyte metabolism and proliferation   during mouse fetal heart development
Dev Cell
  11.800
TREM2 mediates MHCII-associated CD4+ T cell response against gliomas
Neuro Oncol
  15.900
Tumor and immune cell types interact to produce heterogeneous phenotypes of   pediatric high grade glioma
Neuro Oncol
  15.900
Wound-healing plasticity enables clonal expansion of founder progenitor cells in   colitis
Dev Cell
  11.800
A dual-responsive ratiometric indicator designed for in vivo monitoring of   oxidative stress and antioxidant capacity
Chem Sci
   8.400
A heritable iron memory enables decision-making in Escherichia coli
Proc Natl Acad Sci U S A
  11.100
Aging impairs the ability of vascular endothelial stem cells to generate   endothelial cells in mice
Angiogenesis
   9.800
Autophagy critically controls skin inflammation and apoptosis-induced stem cell   activation
Autophagy
  13.300
Baseline immune states (BIS) associated with vaccine responsiveness and factors   that shape the BIS
Semin Immunol
   7.800
Controlling donor and newborn neuron migration and maturation in the eye through   microenvironment engineering
Proc Natl Acad Sci U S A
  11.100
Cross-talk between Myeloid and B Cells Shapes the Distinct Microenvironments of   Primary and Secondary Liver Cancer
Cancer Res
  11.200
Expressed Barcoding Enables High-Resolution Tracking of the Evolution of Drug   Tolerance
Cancer Res
  11.200
Fasting-Mimicking Diet Drives Antitumor Immunity against Colorectal Cancer by   Reducing IgA-Producing Cells
Cancer Res
  11.200
Fluid dynamics alters liquid-liquid phase separation in confined aqueous   two-phase systems
Proc Natl Acad Sci U S A
  11.100
GPCR signaling contributes to immune characteristics of microenvironment and   process of EBV-induced lymphomagenesis
Sci Bull (Beijing)
  18.900
High-dimensional single-cell proteomics analysis of esophageal squamous cell   carcinoma reveals dynamic alterations of the tumor immune microenvironment after   neoadjuvant therapy
J Immunother Cancer
  10.900
Loss of ADAR1 in macrophages in combination with interferon gamma suppresses   tumor growth by remodeling the tumor microenvironment
J Immunother Cancer
  10.900
MDR1-EXPRESSING CD4(+) T CELLS WITH TH1.17 FEATURES RESIST TO NEOADJUVANT   CHEMOTHERAPY AND ARE ASSOCIATED WITH BREAST CANCER CLINICAL RESPONSE
J Immunother Cancer
  10.900
Melatonin alleviates the toxic effect of di(2-ethylhexyl) phthalate on oocyte   quality resulting from CEBPB suppression during primordial follicle formation
J Hazard Mater
  13.600
Minorities drive growth resumption in cross-feeding microbial communities
Proc Natl Acad Sci U S A
  11.100
MLKL deficiency alleviates neuroinflammation and motor deficits in the   伪-synuclein transgenic mouse model of Parkinson's disease
Mol Neurodegener
  15.100
Neurotransmitter Receptor HTR2B Regulates Lipid Metabolism to Inhibit Ferroptosis   in Gastric Cancer
Cancer Res
  11.200
NMDAR antagonists suppress tumor progression by regulating tumor-associated   macrophages
Proc Natl Acad Sci U S A
  11.100
On-capillary alkylation micro-reactor: a facile strategy for proteo-metabolome   profiling in the same single cells
Chem Sci
   8.400
One mutation to rule them all: Mutant KRAS controls tumor intrinsic and   microenvironment signaling
Cancer Res
  11.200
Single-cell bisulfite-free 5mC and 5hmC sequencing with high sensitivity and   scalability
Proc Natl Acad Sci U S A
  11.100
Single-cell dynamics of liver development in postnatal pigs
Sci Bull (Beijing)
  18.900
Single-cell insights into epithelial morphogenesis in the neonatal mouse uterus
Proc Natl Acad Sci U S A
  11.100
Single-cell RNA-sequencing atlas reveals an FABP1-dependent immunosuppressive   environment in hepatocellular carcinoma
J Immunother Cancer
  10.900
Single-cell transcriptomics reveals the pulmonary inflammation induced by   inhalation of subway fine particles
J Hazard Mater
  13.600
SPA, a Stigma-style-transmitting tract Physical microenvironment Assay for   investigating mechano-signaling in pollen tubes
Proc Natl Acad Sci U S A
  11.100
Spatial and single-cell transcriptomics reveal a cancer-associated fibroblast   subset in HNSCC that restricts infiltration and anti-tumor activity of CD8+ T   cells
Cancer Res
  11.200
Targeting integrin 伪5 in fibroblasts potentiates colorectal cancer response to   PD-L1 blockade by affecting extracellular-matrix deposition
J Immunother Cancer
  10.900
Targeting myeloid checkpoint Siglec-10 reactivates antitumor immunity and   improves anti-programmed cell death 1 efficacy in gastric cancer
J Immunother Cancer
  10.900
Unraveling T-cell Exhaustion: Genetic Screening Meets In Vitro Modeling
Cancer Res
  11.200

文章来源于公众号-序科码
编辑:许雪琪,林子翔,韩金,孙志豪,张银冰,张启蒙,黄冬雯,罗雨馨,董小媚,贾鑫淼,刘娴阳,李行,丁怡雯,郑泽鑫,徐荣
排版:张启蒙

-end-
加入单细胞行业交流群,请关注公众号回复“进群



分享到:
关于单细胞测序网
单细胞测序网以“加速单细胞测序产业转化”为核心理念,打造聚焦单细胞测序及时空组学领域的资讯和产业服务平台。我们与全国上百家医疗机构、科研机构、专业协会、第三方实验室以及单细胞测序科技企业建立紧密合作关系,以专业深度的视角,给行业从业人员传递最新最全的科研资讯和产业信息。
微信公众号
友情链接:单细胞测序网  早筛网